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Rat Solute Carrier Family 30, Member 1 (SLC30A1) ELISA Kit

SKU:
RTDL00959
Product Type:
ELISA Kit
Sensitivity:
32pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
ZNT1,ZRC1,Zinc Transporter 1
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$899
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Description

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Rat Solute Carrier Family 30, Member 1 (SLC30A1) ELISA Kit

The Rat Solute Carrier Family 30 Member 1 (SLC30A1) ELISA Kit is a powerful tool for detecting levels of SLC30A1 in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, providing accurate and consistent results for a variety of research applications.SLC30A1 is a key transporter protein responsible for zinc transportation across cell membranes, playing a critical role in zinc homeostasis and cellular function. Dysregulation of SLC30A1 has been implicated in various diseases, including diabetes, Alzheimer's disease, and cancer, making it a valuable target for understanding disease mechanisms and developing potential treatments.

With the Rat SLC30A1 ELISA Kit, researchers can confidently analyze SLC30A1 levels in rat samples, enabling further exploration of its role in disease pathogenesis and therapeutic interventions. Trust in this kit to provide reliable data and valuable insights for your research endeavors.

Product Name:Rat Solute Carrier Family 30, Member 1 (SLC30A1) ELISA Kit
Product Code:RTDL00959
Size:96 Assays
Target:SLC30A1
Synonyms:ZNT1, ZRC1, Zinc Transporter 1
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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