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Rat Solute Carrier Family 16, Member 1 (SLC16A1) ELISA Kit

SKU:
RTDL00958
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P53987
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MCT1,MCT,Monocarboxylate Transporter 1,Monocarboxylic Acid Transporter 1
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Rat Solute Carrier Family 16, Member 1 (SLC16A1) ELISA Kit

The Rat Solute Carrier Family 16 Member 1 (SLC16A1) ELISA Kit is a powerful tool for measuring levels of this key transporter protein in rat samples. SLC16A1 is involved in the transport of monocarboxylates such as lactate and pyruvate, and is crucial for maintaining cellular metabolism and energy production.This ELISA kit offers high sensitivity and specificity, allowing for accurate and reliable measurements of SLC16A1 levels in rat serum, plasma, and cell culture supernatants.

By accurately quantifying SLC16A1 levels, researchers can gain valuable insights into metabolic processes, as well as potential implications in diseases such as diabetes, cancer, and cardiovascular disorders.Overall, the Rat SLC16A1 ELISA Kit is an essential tool for researchers studying metabolism, cellular transport, and disease mechanisms in rats, providing valuable data for further research and potential therapeutic developments.

Product Name:Rat Solute Carrier Family 16, Member 1 (SLC16A1) ELISA Kit
Product Code:RTDL00958
Size:96 Assays
Target:SLC16A1
Synonyms:MCT1, MCT, Monocarboxylate Transporter 1, Monocarboxylic Acid Transporter 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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