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Rat SLC (Secondary Lymphoid Tissue Chemokine) ELISA Kit (AEES00483)

SKU:
AEES00483
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
37.50 pg/mL
Range:
62.50-4000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
$719

Description

Rat SLC (Secondary Lymphoid Tissue Chemokine) ELISA Kit

The Rat SLC (Secondary Lymphoid Tissue Chemokine) ELISA Kit is a specialized assay designed for the quantitative detection of Secondary Lymphoid Tissue Chemokine levels in various rat biological samples. Secondary Lymphoid Tissue Chemokine plays a critical role as a chemokine in the immune system, regulating the migration and activity of lymphocytes and dendritic cells. It is involved in the formation of secondary lymphoid organs and the immune response to infections and inflammatory conditions. This ELISA kit provides a precise and reliable method for quantifying Secondary Lymphoid Tissue Chemokine levels, enabling researchers to study its role in immune cell trafficking and inflammation.

With exceptional sensitivity and specificity, this assay ensures accurate and reproducible results. Manufactured under stringent quality control standards, the Rat SLC ELISA Kit offers robust performance and ease of use, making it an ideal choice for immunology research and investigations into the immune system's chemotactic responses.

Product Name:Rat SLC (Secondary Lymphoid Tissue Chemokine) ELISA Kit
Product Code:AEES00483
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:62.50-4000 pg/mL
Sensitivity:37.50 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat SLC in samples. No significant cross-reactivity or interference between Rat SLC and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat SLC concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SLC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SLC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SLC. You can calculate the concentration of Rat SLC in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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