Rat SIRT1 (NAD-dependent protein deacetylase sirtuin-1) ELISA Kit
The Rat SIRT1 (NAD-dependent protein deacetylase sirtuin 1) ELISA Kit is a reliable and accurate tool for measuring the levels of SIRT1 in rat serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results for a variety of research applications.SIRT1 is a critical enzyme involved in regulating various cellular processes, including metabolism, DNA repair, and stress response.
It plays a crucial role in aging, inflammation, and metabolic diseases, making it a valuable target for studying these conditions and developing therapeutic interventions.With the Rat SIRT1 ELISA Kit, researchers can easily quantify SIRT1 levels in rat samples, providing important insights into its biological functions and potential implications for human health.
Product Name:
Rat SIRT1 (NAD-dependent protein deacetylase sirtuin-1) ELISA Kit
SKU:
RTES00908
Target:
Rat SIRT1 (NAD-dependent protein deacetylase sirtuin-1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SIRT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SIRT1. You can calculate the concentration of Rat SIRT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
88-100
95-112
Average (%)
103
95
102
1:4
Range (%)
90-103
87-98
84-96
Average (%)
97
93
91
1:8
Range (%)
91-102
83-96
84-98
Average (%)
96
89
90
1:16
Range (%)
90-102
87-101
83-94
Average (%)
97
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
92-107
97
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.51
3.5
0.5
1.61
3.79
Standard deviation
0.04
0.08
0.12
0.03
0.07
0.13
C V (%)
7.69
5.3
3.43
6.0
4.35
3.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SIRT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SIRT1 in samples. No significant cross-reactivity or interference between Rat SIRT1 and analogues was observed.
