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Rat Signal Transducer And Activator Of Transcription 4 (STAT4) ELISA Kit

SKU:
RTDL00997
Product Type:
ELISA Kit
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
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Description

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Rat Signal Transducer And Activator Of Transcription 4 (STAT4) ELISA Kit

The Rat Signal Transducer and Activator of Transcription 4 (STAT4) ELISA Kit is a powerful tool for researchers studying STAT4 signaling pathways in rats. This kit is specifically designed to accurately measure levels of STAT4 in rat serum, plasma, and tissue samples with high sensitivity and specificity.STAT4 is a key transcription factor that plays a critical role in the regulation of immune responses, particularly in the development of T helper 1 (Th1) cells.

Dysregulation of STAT4 signaling has been implicated in various autoimmune diseases, making it a valuable target for research and drug development.With its easy-to-use protocol and precise results, the Rat STAT4 ELISA Kit is an essential tool for studying the role of STAT4 in inflammation, autoimmune diseases, and other immune-related disorders in rats. Get reliable and reproducible data to advance your research with this innovative ELISA kit.

Product Name:Rat Signal Transducer And Activator Of Transcription 4 (STAT4) ELISA Kit
Product Code:RTDL00997
Size:96 Assays
Target:STAT4
Detection Method:Sandwich
Range:78.12-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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