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Rat Sex Hormone Binding Globulin (SHBG) ELISA Kit

SKU:
RTDL00952
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08689
Sensitivity:
6.7pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
ABP,TEBG,SBP,Sex steroid-binding protein,Testis-specific androgen-binding protein,Testosterone-estradiol-binding globulin,Testosterone-estrogen-binding globulin
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Sex Hormone Binding Globulin (SHBG) ELISA Kit

The Rat Sex Hormone Binding Globulin (SHBG) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of SHBG levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.SHBG is a key protein that binds to sex hormones such as testosterone and estradiol, regulating their bioavailability and activity. Changes in SHBG levels have been linked to various hormonal disorders, metabolic diseases, and reproductive health issues.

Studying SHBG levels can provide valuable insights into these conditions and potential therapeutic targets.Overall, the Rat SHBG ELISA Kit offers researchers a powerful tool for investigating the role of SHBG in physiological and pathological processes, leading to a deeper understanding of hormone regulation and potential therapeutic interventions.

Product Name:Rat Sex Hormone Binding Globulin (SHBG) ELISA Kit
Product Code:RTDL00952
Size:96 Assays
Target:SHBG
Synonyms:ABP, TEBG, SBP, Sex steroid-binding protein, Testis-specific androgen-binding protein, Testosterone-estradiol-binding globulin, Testosterone-estrogen-binding globulin
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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