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Rat Semaphorin 3A (SEMA3A) ELISA Kit

SKU:
RTDL00941
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q63548
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SEMAL,Hsema-I,Hsema-III,SEMA1,SEMAD,SEMAIII,SemD,Coll-1,Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Rat Semaphorin 3A (SEMA3A) ELISA Kit

The Rat Semaphorin 3A (Sema3A) ELISA Kit is specially designed for the precise measurement of semaphorin 3A levels in rat serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit guarantees accurate and consistent results, making it an invaluable tool for various research purposes.Sema3A is a key protein involved in neural development and axon guidance, playing a crucial role in the central nervous system. Dysregulation of semaphorin 3A has been linked to neurological disorders such as autism, schizophrenia, and Alzheimer's disease, making it a crucial biomarker for studying these conditions and exploring potential therapeutic interventions.

With the Rat Semaphorin 3A ELISA Kit, researchers can accurately measure semaphorin 3A levels in biological samples, providing valuable insights into its role in neurodevelopmental processes and neurological disorders.

Product Name:Rat Semaphorin 3A (SEMA3A) ELISA Kit
Product Code:RTDL00941
Size:96 Assays
Target:SEMA3A
Synonyms:SEMAL, Hsema-I, Hsema-III, SEMA1, SEMAD, SEMAIII, SemD, Coll-1, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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