The Rat Selenoprotein P1 (Plasma SEPP1) ELISA Kit is a powerful tool for the accurate quantification of SEPP1 levels in rat plasma samples. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications.SEPP1 is a critical selenoprotein that plays a key role in regulating selenium metabolism and antioxidant defense in the body. It is involved in various biological processes, including immune function, thyroid hormone regulation, and protection against oxidative stress.
Changes in SEPP1 levels have been linked to conditions such as cardiovascular diseases, neurological disorders, and cancer, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.Overall, the Rat SEPP1 ELISA Kit is an essential tool for researchers interested in investigating the role of SEPP1 in health and disease, providing valuable insights into its function and potential clinical applications.
Product Name:
Rat Selenoprotein P1, Plasma (SEPP1) ELISA Kit
Product Code:
RTDL00942
Size:
96 Assays
Target:
SEPP1
Synonyms:
SEPP, SeP, SEP-P1, SELP
Detection Method:
Sandwich
Range:
6.25-400ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
