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Rat Selectin, Platelet (SELP) ELISA Kit

SKU:
RTDL00940
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P98106
Sensitivity:
0.138ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD62P,P-Selectin,PSEL,GMP140,LECAM3,GRMP,GMRP,PADGEM,Leukocyte-endothelial cell adhesion molecule 3,Platelet activation dependent granule-external membrane
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat Selectin, Platelet (SELP) ELISA Kit

The Rat Platelet Selectin (SELP) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of SELP levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.SELP, also known as P-Selectin, is a cell adhesion molecule that plays a crucial role in the recruitment of leukocytes to sites of inflammation and tissue injury. Its expression is upregulated in various inflammatory conditions, making it a valuable biomarker for studying these processes and developing potential therapeutic interventions.

Overall, the Rat Platelet Selectin (SELP) ELISA Kit is a valuable tool for researchers studying inflammation, immune responses, and thrombotic disorders in rat models. Its high sensitivity and accuracy make it a reliable option for investigating the role of SELP in various physiological and pathological processes.

Product Name:Rat Selectin, Platelet (SELP) ELISA Kit
Product Code:RTDL00940
Size:96 Assays
Target:SELP
Synonyms:CD62P, P-Selectin, PSEL, GMP140, LECAM3, GRMP, GMRP, PADGEM, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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