The Rat Secretory Immunoglobulin A (sIgA) ELISA Kit is a reliable tool for quantitative detection of secretory immunoglobulin A levels in rat biological samples such as serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for your research needs.Secretory immunoglobulin A (sIgA) is a vital component of the immune system, defending against pathogens at mucosal surfaces and providing essential protection against infections.
Monitoring sIgA levels can provide insights into immune function and response to various stimuli, making it a valuable biomarker for studying immune-related disorders and therapeutic interventions.Whether you are investigating immune responses in infectious diseases, inflammatory conditions, or gut health, the Rat Secretory Immunoglobulin A (sIgA) ELISA Kit is a valuable tool for advancing your research and understanding the role of sIgA in maintaining immune homeostasis.
Product Name:
Rat Secretory Immunoglobulin A (sIgA) ELISA Kit
Product Code:
RTDL00955
Size:
96 Assays
Target:
sIgA
Synonyms:
S-IgA
Detection Method:
Sandwich
Range:
0.94-60ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
85-103
94
Heparin plasma (n=5)
87-99
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
