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Rat Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit

SKU:
RTDL00946
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JLS4
Sensitivity:
0.06ng/mL
Range:
0.187-12ng/mL
ELISA Type:
Sandwich
Synonyms:
FRP-4,FRPHE,Frizzled protein, human endometrium
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Rat Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit

The Rat Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit is specifically designed for the accurate and reliable detection of SFRP4 levels in rat serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring consistent and precise results for a variety of research applications.SFRP4 is a key protein that is involved in regulating the Wnt signaling pathway, which plays a critical role in cell proliferation, development, and differentiation.

Dysregulation of Wnt signaling has been implicated in various diseases, including cancer, metabolic disorders, and skeletal diseases, making SFRP4 a valuable biomarker for studying these conditions.The Rat SFRP4 ELISA Kit provides researchers with a powerful tool to accurately measure SFRP4 levels in biological samples, allowing for in-depth investigations into the role of this protein in disease pathogenesis and the development of potential therapeutic strategies.

Product Name:Rat Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit
Product Code:RTDL00946
Size:96 Assays
Target:SFRP4
Synonyms:FRP-4, FRPHE, Frizzled protein, human endometrium
Detection Method:Sandwich
Range:0.187-12ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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