Rat SDF-1/CXCL12 (Stromal Cell Derived Factor 1) ELISA Kit
The Rat Stromal Cell-Derived Factor 1 (SDF-1/CXCL12) ELISA Kit is a specialized assay meticulously developed for the precise quantitative measurement of SDF-1 levels in various biological samples derived from rat models. SDF-1, also known as CXCL12, is a potent chemokine that plays a crucial role in cell migration, homing, and differentiation, exerting its functions by interacting with its cognate receptor CXCR4. This ELISA kit empowers researchers to accurately quantify SDF-1 concentrations, providing valuable insights into its involvement in diverse physiological and pathological processes such as stem cell mobilization, hematopoiesis, angiogenesis, and inflammation.
By quantifying SDF-1 levels with high sensitivity and specificity, this kit enables researchers to elucidate the dynamic roles of SDF-1/CXCL12 in various biological systems. Manufactured under stringent quality control standards, the Rat SDF-1/CXCL12 ELISA Kit ensures robust performance and reproducible results, allowing researchers to explore the intricate role of SDF-1 in a wide array of cellular activities. With its exceptional sensitivity and reliability, this kit is an excellent choice for research studies focusing on the intricate mechanisms of cell migration, signaling, and tissue homeostasis in rat models.
Product Name:
Rat SDF-1/CXCL12 (Stromal Cell Derived Factor 1) ELISA Kit
SKU:
AEES00541
Target:
Rat SDF-1/CXCL12 (Stromal Cell Derived Factor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SDF-1/CXCL12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDF-1/CXCL12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SDF-1/CXCL12. You can calculate the concentration of Rat SDF-1/CXCL12 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
82-97
97-107
82-96
Average (%)
90
104
90
1:4
Range (%)
91-98
89-96
92-101
Average (%)
94
93
98
1:8
Range (%)
84-97
100-106
89-97
Average (%)
90
103
94
1:16
Range (%)
92-103
96-104
89-100
Average (%)
97
100
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
103
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
83-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.89
2.29
8.82
0.93
2.07
8.78
Standard deviation
0.05
0.11
0.39
0.05
0.1
0.47
C V (%)
5.62
4.8
4.42
5.38
4.83
5.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Rat SDF-1/CXCL12 and analogues was observed.
