Rat SCD86 (Soluble Cluster of Differentiation 41 Ligand) ELISA Kit
The Rat SCD86 (Soluble Cluster of Differentiation 86) ELISA Kit is specially designed for the precise quantification of soluble cluster of differentiation 86 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Soluble cluster of differentiation 86, also known as CD86, is a key protein involved in immune response regulation and T-cell activation. It plays a crucial role in modulating immune responses and is implicated in autoimmune diseases, allergy, and transplant rejection.
The Rat SCD86 ELISA Kit provides a valuable tool for studying the role of CD86 in these conditions and exploring potential therapeutic interventions.Overall, the Rat SCD86 ELISA Kit is an indispensable tool for researchers investigating the immune system, inflammatory disorders, and autoimmune diseases in rat models. Its reliability and precision make it an essential addition to any laboratory conducting research in these areas.
Product Name:
Rat SCD86 (Soluble Cluster of Differentiation 41 Ligand) ELISA Kit
SKU:
RTES00743
Target:
Rat SCD86 (Soluble Cluster of Differentiation 41 Ligand)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sCD86. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sCD86 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sCD86, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat sCD86. You can calculate the concentration of Rat sCD86 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
94-110
90-106
Average (%)
96
101
98
1:4
Range (%)
92-107
81-95
88-101
Average (%)
98
88
93
1:8
Range (%)
90-103
83-95
87-102
Average (%)
98
89
93
1:16
Range (%)
94-107
82-95
90-103
Average (%)
99
89
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
95
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2
4.28
16.82
2.01
3.92
17.72
Standard deviation
0.1
0.18
0.79
0.11
0.21
0.8
C V (%)
5
4.21
4.7
5.47
5.36
4.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat sCD86 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat sCD86 in samples. No significant cross-reactivity or interference between Rat sCD86 and analogues was observed.
