The Rat Serum Amyloid A (SAA) ELISA Kit is a specialized assay meticulously designed to quantitatively measure the levels of SAA in various biological samples from rat models.
SAA, as an acute-phase protein, is known for its rapid and marked increase in response to inflammatory stimuli, acting as a key player in the acute phase response. Elevated SAA levels are associated with various conditions such as inflammation, infections, and other inflammatory disorders, making it a valuable biomarker for assessing inflammatory states and disease progression. This ELISA kit enables researchers to accurately quantify SAA concentrations, facilitating investigations into the dynamics of inflammation, immune responses, and disease pathologies in rat models. By measuring SAA levels with precision, researchers can gain valuable insights into the inflammatory processes and immune system activation, thereby aiding in the understanding of various disease mechanisms and the development of potential therapeutic interventions. Manufactured under stringent quality control standards, the Rat SAA ELISA Kit ensures exceptional sensitivity and specificity, resulting in reliable and reproducible data. With its robust performance and user-friendly design, this kit is an excellent choice for research studies focusing on inflammation, immune responses, and related disorders in rat models, providing valuable data for scientific investigations.
Product Name:
Rat SAA (Serum Amyloid A) ELISA Kit
SKU:
AEES00540
Target:
Rat SAA (Serum Amyloid A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SAA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SAA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SAA. You can calculate the concentration of Rat SAA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-95
98-111
90-105
Average (%)
92
105
98
1:4
Range (%)
88-95
97-111
94-107
Average (%)
91
105
100
1:8
Range (%)
95-101
86-101
92-101
Average (%)
98
94
97
1:16
Range (%)
86-101
98-110
102-110
Average (%)
93
104
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-105
99
EDTA plasma (n=5)
88-99
91
Cell culture media (n=5)
89-100
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.62
12.36
49.07
4.73
12.21
48.15
Standard deviation
0.26
0.56
2.48
0.24
0.6
2.52
C V (%)
5.63
4.53
5.05
5.07
4.91
5.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SAA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SAA in samples. No significant cross-reactivity or interference between Rat SAA and analogues was observed.
