Rat S100A11 (S100 Calcium Binding Protein A11) CLIA Kit
The Rat S100A11 (S100 Calcium Binding Protein A11) CLIA Kit is a highly sensitive and specific assay designed for the quantitative measurement of S100A11 levels in rat samples including serum, plasma, and tissue lysates. This kit provides accurate and reproducible results, making it a valuable tool for studying the role of S100A11 in various research applications.S100A11 is a member of the S100 protein family known for its calcium-binding properties and involvement in cell proliferation, differentiation, and inflammation.
Elevated levels of S100A11 have been linked to various diseases such as cancer, cardiovascular disorders, and inflammatory conditions, making it an important biomarker for understanding disease progression and potential therapeutic strategies.Overall, the Rat S100A11 CLIA Kit offers researchers a reliable and efficient way to analyze S100A11 levels in rat samples, providing valuable insights into the role of this protein in disease pathogenesis and potential treatment options.
Product Name:
Rat S100A11 (S100 Calcium Binding Protein A11) CLIA Kit
SKU:
RTES00504
Target:
Rat S100A11 (S100 Calcium Binding Protein A11)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat S100A11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat S100A11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat S100A11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat S100A11. You can calculate the concentration of Rat S100A11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
94-105
85-98
Average (%)
109
100
92
1:4
Range (%)
93-107
95-109
101-118
Average (%)
101
101
109
1:8
Range (%)
93-107
102-115
86-100
Average (%)
100
108
91
1:16
Range (%)
92-106
99-114
88-103
Average (%)
98
105
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
105
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.11
2.11
6.84
1.18
1.98
7.41
Standard deviation
0.12
0.22
0.42
0.15
0.2
0.45
C V (%)
10.81
10.43
6.14
12.71
10.1
6.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat S100A11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat S100A11 in samples. No significant cross-reactivity or interference between Rat S100A11 and analogues was observed.
