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Rat S100 Calcium Binding Protein A4 (S100A4) ELISA Kit

SKU:
RTDL00920
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05942
Sensitivity:
34pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
S100-A4,MTS1,18A2,42A,CAPL,P9KA,PEL98,Calcium protein,Calvasculin,Metastasin,Protein Mts1,Placental calcium-binding protein,Murine placental homolog
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Rat S100 Calcium Binding Protein A4 (S100A4) ELISA Kit

The Rat S100 Calcium Binding Protein A4 (S100A4) ELISA Kit is a powerful tool designed for the precise measurement of S100A4 levels in rat serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.S100A4 is a key calcium-binding protein that plays a crucial role in cell migration, invasion, and metastasis in various pathological conditions, including cancer and inflammatory diseases.

By quantifying S100A4 levels, researchers can gain valuable insights into disease progression and potential therapeutic targets.With its user-friendly protocol and reliable performance, the Rat S100A4 ELISA Kit is an indispensable tool for studying the biology and pathology of S100A4, providing researchers with the opportunity to advance their understanding of complex disease mechanisms and develop novel treatment strategies.

Product Name:Rat S100 Calcium Binding Protein A4 (S100A4) ELISA Kit
Product Code:RTDL00920
Size:96 Assays
Target:S100A4
Synonyms:S100-A4, MTS1, 18A2, 42A, CAPL, P9KA, PEL98, Calcium protein, Calvasculin, Metastasin, Protein Mts1, Placental calcium-binding protein, Murine placental homolog
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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