The Rat RETN (Resistin) ELISA Kit is a highly specialized assay designed to quantitatively measure levels of Resistin in various biological samples of rat origin. Resistin is a significant cytokine involved in metabolic processes, inflammation, and the regulation of insulin sensitivity, making it a crucial factor in understanding metabolic disorders and associated diseases.
This advanced ELISA kit from Assay Genie offers unparalleled sensitivity and specificity, facilitating accurate and reproducible results in your research. Manufactured under strict quality control standards, this ELISA kit ensures robust performance, guaranteeing the efficiency and reliability needed for in-depth investigations into the role of Resistin in metabolic and inflammatory pathways. Trust in Assay Genie's ELISA Kit for comprehensive and precise measurement of Resistin levels, advancing your research endeavors with confidence.
Product Name:
Rat RETN (Resistin) ELISA Kit
SKU:
AEES00476
Target:
Rat RETN (Resistin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat RETN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RETN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat RETN. You can calculate the concentration of Rat RETN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
85-98
90-106
Average (%)
95
91
97
1:4
Range (%)
96-113
85-95
94-108
Average (%)
103
90
100
1:8
Range (%)
94-108
88-101
94-109
Average (%)
100
93
101
1:16
Range (%)
97-111
82-97
92-107
Average (%)
105
89
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
100
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
89-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.3
7.9
1.1
2.1
7.2
Standard deviation
0.1
0.1
0.4
0.1
0.1
0.4
C V (%)
10
4.35
5.06
9.09
4.76
5.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat RETN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat RETN in samples. No significant cross-reactivity or interference between Rat RETN and analogues was observed.
