The Rat Retinoic Acid Receptor Alpha (RARA) ELISA Kit is a top-quality assay kit designed for the accurate and precise detection of RARA levels in rat samples. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Retinoic Acid Receptor Alpha is a key nuclear receptor that plays a crucial role in regulating gene expression, cell differentiation, and development. Dysregulation of RARA has been linked to numerous diseases, including cancer, autoimmune disorders, and developmental abnormalities.
Therefore, measuring RARA levels can provide valuable insights into disease mechanisms and potential treatment options.With easy-to-follow instructions and quick assay protocols, the Rat RARA ELISA Kit is perfect for researchers looking to study the role of RARA in various biological processes. Invest in this kit today to accelerate your research and make significant advancements in the field of molecular biology.
Product Name:
Rat Retinoic Acid Receptor Alpha (RARa) ELISA Kit
Product Code:
RTDL00897
Size:
96 Assays
Target:
RARa
Synonyms:
RAR-A, NR1B1, RAR, RAR-Alpha, Nuclear Receptor Subfamily 1 Group B Member 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
