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Rat RegeneRat ing Islet Derived Protein 1 Alpha (REG1a) ELISA Kit

SKU:
RTDL00899
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P10758
Sensitivity:
12.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
REG,PSP,ICRF,PSPS1,PTP,Islet cells regeneration factor,Islet of Langerhans regenerating protein,Lithostathine 1 Alpha,Pancreatic Stone Protein,Pancreatic Thread Protein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

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Rat RegeneRat ing Islet Derived Protein 1 Alpha (REG1a) ELISA Kit

The Rat Regenerating Islet-Derived Protein 1 Alpha (REG1A) ELISA Kit is specifically designed for the precise quantification of REG1A levels in rat serum, plasma, and tissue homogenates. This user-friendly kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for a variety of research applications.REG1A, also known as Regenerating Islet-Derived Protein 1 Alpha, is a key factor in promoting pancreatic islet regeneration and beta cell proliferation.

It plays a crucial role in regulating glucose metabolism and may hold potential implications for diabetes research and therapy development.With its easy-to-use format and reliable performance, the Rat REG1A ELISA Kit is an essential tool for researchers studying pancreatic regeneration, diabetes, and related metabolic disorders. Accelerate your research with this cutting-edge kit and unlock new insights into REG1A biology.

Product Name:Rat RegeneRat ing Islet Derived Protein 1 Alpha (REG1a) ELISA Kit
Product Code:RTDL00899
Size:96 Assays
Target:REG1a
Synonyms:REG, PSP, ICRF, PSPS1, PTP, Islet cells regeneration factor, Islet of Langerhans regenerating protein, Lithostathine 1 Alpha, Pancreatic Stone Protein, Pancreatic Thread Protein
Detection Method:Sandwich
Range:31.2-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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