The Rat PYY (Peptide YY) ELISA Kit is specifically designed to enable precise and quantitative detection of Peptide YY levels in a variety of rat biological samples.
Peptide YY, an important gastrointestinal hormone, plays a crucial role in appetite regulation, energy metabolism, and gastrointestinal motility. It is involved in mediating satiety signals, reducing food intake, and modulating metabolic responses related to digestion and energy balance. Accurate measurement of Peptide YY levels using this ELISA kit provides valuable insights into the regulatory mechanisms of appetite control, energy homeostasis, and gastrointestinal functions. Understanding the role of Peptide YY in physiological processes is essential for investigating its impact on metabolic disorders, obesity, and related health conditions. By precisely quantifying Peptide YY levels, researchers can gain a deeper understanding of its functions and explore potential therapeutic strategies targeting these pathways. The Rat PYY (Peptide YY) ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures robust performance and ease of use, making it an ideal choice for cutting-edge research in the fields of metabolism, nutrition, and digestive health.
Product Name:
Rat PYY (Peptide YY) ELISA Kit
SKU:
AEES00504
Target:
Rat PYY (Peptide YY)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PYY. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PYY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PYY, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PYY. You can calculate the concentration of Rat PYY in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
89-101
95-110
Average (%)
103
94
103
1:4
Range (%)
88-99
83-93
85-96
Average (%)
94
88
90
1:8
Range (%)
91-106
79-90
82-93
Average (%)
98
85
88
1:16
Range (%)
89-104
87-102
81-92
Average (%)
96
93
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.17
114.96
399.44
47.83
126.28
430.38
Standard deviation
3.45
5.85
21.57
2.77
5.59
13.08
C V (%)
6.61
5.09
5.4
5.79
4.43
3.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PYY concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PYY in samples. No significant cross-reactivity or interference between Rat PYY and analogues was observed.
