null

Rat Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) ELISA Kit (RTDL00817)

SKU:
RTDL00817
Product Type:
ELISA Kit
Sensitivity:
33.2pg/mL
Range:
78.1-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

Rat Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) ELISA Kit

The Rat Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) ELISA Kit is specially designed for accurate detection of PDK4 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for various research applications.PDK4 is an important enzyme that plays a key role in regulating energy metabolism by controlling the activity of pyruvate dehydrogenase.

Dysregulation of PDK4 has been linked to metabolic disorders such as diabetes, obesity, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Rat PDK4 ELISA Kit provides researchers with a reliable tool for studying the role of PDK4 in metabolic pathways and its implications in various diseases.

Product Name:Rat Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) ELISA Kit
Product Code:RTDL00817
Size:96 Assays
Target:PDK4
Synonyms:[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
Detection Method:Sandwich
Range:78-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products