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Rat Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2) ELISA Kit

SKU:
RTDL00816
Product Type:
ELISA Kit
Sensitivity:
35.6pg/mL
Range:
78.1-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
PDK P45,Pyruvate dehydrogenase kinase isoform 2,PDH kinase 2
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

Rat Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2) ELISA Kit

The Rat Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2) ELISA Kit is specifically designed for the quantitative detection of PDK2 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PDK2 is a key enzyme involved in the regulation of pyruvate dehydrogenase complex, playing a critical role in glucose metabolism and energy production.

Dysregulation of PDK2 has been linked to metabolic disorders such as diabetes and obesity, making it a valuable target for studying these conditions and developing potential therapies.Overall, the Rat PDK2 ELISA Kit provides researchers with a reliable tool for studying the role of PDK2 in metabolic pathways and its potential implications in various diseases.

Product Name:Rat Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2) ELISA Kit
Product Code:RTDL00816
Size:96 Assays
Target:PDK2
Synonyms:PDK P45, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2
Detection Method:Sandwich
Range:78-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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