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Rat Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) ELISA Kit

SKU:
RTDL00884
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P41499
Sensitivity:
0.107ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
BPTP3,CFC,NS1,PTP-1D,PTP2C,SH-PTP2,SH-PTP3,SHP2,Noonan Syndrome 1,Protein-tyrosine phosphatase 1D,Protein-tyrosine phosphatase 2C
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Rat Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) ELISA Kit

The Rat Protein Tyrosine Phosphatase Non-Receptor Type 11 (PTPN11) ELISA Kit is a reliable tool for the accurate detection of PTPN11 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and precise results for various research applications.PTPN11, also known as SHP2, is a critical enzyme involved in cell signaling pathways and regulation. Dysregulation of PTPN11 has been implicated in various diseases, including cancer, developmental disorders, and autoimmune diseases.

Therefore, studying PTPN11 levels can provide valuable insights into disease mechanisms and potential therapeutic interventions.With its user-friendly protocol and quick assay time, the Rat PTPN11 ELISA Kit is an essential tool for researchers studying cell signaling, disease mechanisms, and potential drug targets in rats. Trust in the accuracy and reliability of this ELISA kit for your research needs.

Product Name:Rat Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) ELISA Kit
Product Code:RTDL00884
Size:96 Assays
Target:PTPN11
Synonyms:BPTP3, CFC, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, Noonan Syndrome 1, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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