PRO-C,VKDPC,PC,Vitamin K Dependent Protein C,Inactivator Of Coagulation Factors Va And VIIIa,Autoprothrombin IIA,Blood coagulation factor XIV,Activation peptide
The Rat C-Pro-C ELISA Kit from Assay Genie is specifically designed for the precise quantification of C-propeptide of type I collagen levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for various research applications. The C-propeptide of type I collagen is a key marker for bone formation and turnover, serving as a crucial indicator of bone health and remodeling processes. It plays a vital role in evaluating bone diseases, bone mineral density, and fracture risk, making it an essential tool for researchers studying bone metabolism and skeletal disorders.
With easy-to-follow instructions and a straightforward assay procedure, the Rat C-Pro-C ELISA Kit provides a convenient solution for researchers looking to investigate bone physiology and assess the impact of various therapeutic interventions on bone health. Upgrade your research capabilities with the Assay Genie Rat C-Pro-C ELISA Kit today!
Product Name:
Rat Protein C (PROC) ELISA Kit
Product Code:
RTDL00868
Size:
96 Assays
Target:
PROC
Synonyms:
PRO-C, VKDPC, PC, Vitamin K Dependent Protein C, Inactivator Of Coagulation Factors Va And VIIIa, Autoprothrombin IIA, Blood coagulation factor XIV, Activation peptide
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
