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Rat Protease, Serine 1 (PRSS1) ELISA Kit (RTDL00870)

SKU:
RTDL00870
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P00762
Sensitivity:
0.245ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
TRP1,TRY1,TRY4,TRYP1,Trypsin 1,Cationic Trypsinogen,Beta-trypsin,Serine protease 1,Trypsin I
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Protease, Serine 1 (PRSS1) ELISA Kit

The Rat Protease Serine 1 (PRSS1) ELISA Kit is specifically designed for the precise measurement of PRSS1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.PRSS1, a serine protease, plays a critical role in the regulation of various biological processes, including digestion and blood coagulation.

Abnormal PRSS1 levels have been associated with pancreatic disorders and other diseases, making it a valuable biomarker for studying these conditions and exploring potential treatment options.With its superior performance and reliability, the Rat PRSS1 ELISA Kit is a valuable tool for researchers seeking to investigate the role of PRSS1 in health and disease in rat models.

Product Name:Rat Protease, Serine 1 (PRSS1) ELISA Kit
Product Code:RTDL00870
Size:96 Assays
Target:PRSS1
Synonyms:TRP1, TRY1, TRY4, TRYP1, Trypsin 1, Cationic Trypsinogen, Beta-trypsin, Serine protease 1, Trypsin I
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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