Rat Prostaglandin G/H synthase 2 (Ptgs2) ELISA Kit (RTEB0412)
- SKU:
- RTEB0412
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P35355
- Range:
- 1.56-100 U/L
- ELISA Type:
- Sandwich
- Synonyms:
- PTGS2, COX-2, Prostaglandin Endoperoxide Synthase 2
- Reactivity:
- Rat
Description
Rat Prostaglandin G/H synthase 2 (Ptgs2) ELISA Kit
The Rat Prostaglandin G/H Synthase 2 (PTGS2) ELISA Kit is specially designed for the accurate quantitative detection of PTGS2 levels in rat serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.PTGS2, also known as cyclooxygenase-2 (COX-2), is an enzyme involved in the production of prostaglandins, which play a crucial role in inflammation and various physiological processes.
Dysregulation of PTGS2 has been linked to inflammatory diseases, cancer, and other disorders, making it a valuable tool for understanding the underlying mechanisms and developing potential therapeutic strategies.With its easy-to-use format and reliable performance, the Rat PTGS2 ELISA Kit is an essential tool for researchers studying inflammation, cancer biology, and drug discovery. Upgrade your research capabilities with this advanced kit and uncover new insights into PTGS2 biology.
| Product Name: | Rat Prostaglandin G/H synthase 2 (Ptgs2) ELISA Kit |
| SKU: | RTEB0412 |
| Size: | 96T |
| Target: | Rat Prostaglandin G/H synthase 2 (Ptgs2) |
| Synonyms: | Cyclooxygenase-2, PHS II, Prostaglandin H2 synthase 2, Prostaglandin-endoperoxide synthase 2, COX-2, PGH synthase 2, Cox-2, Cox2 |
| Assay Type: | Sandwich |
| Detection Method: | ELISA |
| Reactivity: | Rat |
| Detection Range: | 1.56-100U/L |
| Sensitivity: | 0.78U/L |
| Intra CV: | 4.6% | ||||||||||||||||||||
| Inter CV: | 7.4% | ||||||||||||||||||||
| Linearity: |
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| Recovery: |
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| Function: | Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. |
| Uniprot: | P35355 |
| Sample Type: | Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids |
| Specificity: | Natural and recombinant rat Prostaglandin G/H synthase 2 |
| Sub Unit: | Homodimer. |
| Research Area: | Cardiovascular |
| Subcellular Location: | Microsome membrane Peripheral membrane protein Endoplasmic reticulum membrane Peripheral membrane protein |
| Storage: | Please see kit components below for exact storage details |
| Note: | For research use only |
| UniProt Protein Function: | COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family. |
| UniProt Protein Details: | Protein type:EC 1.14.99.1; Lipid Metabolism - arachidonic acid; Oxidoreductase Chromosomal Location of Human Ortholog: 13q21 Cellular Component: caveola; cytoplasm; endoplasmic reticulum; neuron projection; nucleus; protein complex Molecular Function:enzyme binding; heme binding; lipid binding; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; prostaglandin-endoperoxide synthase activity; protein binding; protein homodimerization activity Biological Process: aging; angiogenesis; bone mineralization; brown fat cell differentiation; cyclooxygenase pathway; decidualization; embryo implantation; hair cycle; inflammatory response; learning; memory; negative regulation of apoptosis; negative regulation of calcium ion transport; negative regulation of caspase activity; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of smooth muscle contraction; negative regulation of synaptic transmission, dopaminergic; ovulation; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of fever; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of prostaglandin biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle contraction; positive regulation of synaptic plasticity; positive regulation of synaptic transmission, glutamatergic; positive regulation of vasoconstriction; prostaglandin biosynthetic process; regulation of blood pressure; regulation of cell proliferation; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to fructose stimulus; response to glucocorticoid stimulus; response to lipopolysaccharide; response to lithium ion; response to manganese ion; response to organic cyclic substance; response to organic nitrogen; response to organic substance; response to radiation; response to vitamin D; sensory perception of pain |
| UniProt Code: | P35355 |
| NCBI GenInfo Identifier: | 148747270 |
| NCBI Gene ID: | 29527 |
| NCBI Accession: | NP_058928.3 |
| UniProt Secondary Accession: | P35355,Q64379, Q925V4, |
| Molecular Weight: | 69,164 Da |
| NCBI Full Name: | prostaglandin G/H synthase 2 |
| UniProt Protein Name: | Prostaglandin G/H synthase 2 |
| UniProt Synonym Protein Names: | Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2 |
| Protein Family: | Prostaglandin G/H synthase |
| UniProt Gene Name: | Ptgs2 |
| Component | Quantity (96 Assays) | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | -20°C |
| Lyophilized Standard | 2 | -20°C |
| Sample Diluent | 20ml | -20°C |
| Assay Diluent A | 10mL | -20°C |
| Assay Diluent B | 10mL | -20°C |
| Detection Reagent A | 120µL | -20°C |
| Detection Reagent B | 120µL | -20°C |
| Wash Buffer | 30mL | 4°C |
| Substrate | 10mL | 4°C |
| Stop Solution | 10mL | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.
| Step | |
| 1. | Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C. |
| 2. | Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform. |
| 3. | Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper. |
| 4. | Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C. |
| 5. | Repeat the wash process for five times as conducted in step 3. |
| 6. | Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction. |
| 7. | Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing. |
| 8. | Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters. |
| 9. | After experiment, store all reagents according to the specified storage temperature respectively until their expiry. |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
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