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Rat Prostaglandin Endoperoxide Synthase 1 (PTGS1) ELISA Kit

SKU:
RTDL00877
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q63921
Sensitivity:
0.247ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
COX1,COX3,PCOX1,PGG/HS,PGHS1,PHS1,PTGHS,Cyclooxygenase 1,Prostaglandin H2 Synthase 1,Prostaglandin G/H Synthase And Cyclooxygenase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Prostaglandin Endoperoxide Synthase 1 (PTGS1) ELISA Kit

The Rat Prostaglandin Endoperoxide Synthase-1 (PTGS1) ELISA Kit is specifically designed for the accurate measurement of PTGS1 levels in rat serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, allowing for precise and reliable results that can be used in a variety of research applications.PTGS1, also known as cyclooxygenase-1 (COX-1), is an enzyme that plays a key role in the synthesis of prostaglandins, which are important mediators of inflammation and pain. Dysregulation of PTGS1 has been implicated in various inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.

By utilizing the Rat PTGS1 ELISA Kit, researchers can accurately quantify PTGS1 levels in rat samples, providing valuable insights into the role of this enzyme in inflammation and disease pathology. This kit is a valuable tool for advancing research in areas such as pharmacology, immunology, and molecular biology.

Product Name:Rat Prostaglandin Endoperoxide Synthase 1 (PTGS1) ELISA Kit
Product Code:RTDL00877
Size:96 Assays
Target:PTGS1
Synonyms:COX1, COX3, PCOX1, PGG/HS, PGHS1, PHS1, PTGHS, Cyclooxygenase 1, Prostaglandin H2 Synthase 1, Prostaglandin G/H Synthase And Cyclooxygenase
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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