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Rat Prostaglandin E Synthase, Microsomal (PTGES) ELISA Kit

SKU:
RTDL00876
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JHF3
Sensitivity:
0.068ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MGST1L1,MGST-IV,MGST1-L1,PGES,PIG12,PP102,PP1294,TP53I12,PGE Synthase,Tumor Protein p53 Inducible Protein 12,Glutathione S-Transferase 1-Like 1,MGST1-Like 1
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Prostaglandin E Synthase, Microsomal (PTGES) ELISA Kit

The Rat Prostaglandin E Synthase Microsomal (PTGES) ELISA Kit is a highly accurate tool for detecting PTGES levels in rat serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit provides consistently reliable results for a variety of research purposes.PTGES is an important enzyme involved in the synthesis of prostaglandin E2, a key mediator of inflammation and various physiological processes. Dysregulation of PTGES has been implicated in numerous diseases, including inflammatory disorders, cancer, and reproductive conditions.

Therefore, the PTGES ELISA Kit is a valuable asset for studying these conditions and potential therapeutic interventions.Overall, the Rat Prostaglandin E Synthase Microsomal (PTGES) ELISA Kit is a versatile and essential tool for researchers investigating the role of PTGES in health and disease in rat models.

Product Name:Rat Prostaglandin E Synthase, Microsomal (PTGES) ELISA Kit
Product Code:RTDL00876
Size:96 Assays
Target:PTGES
Synonyms:MGST1L1, MGST-IV, MGST1-L1, PGES, PIG12, PP102, PP1294, TP53I12, PGE Synthase, Tumor Protein p53 Inducible Protein 12, Glutathione S-Transferase 1-Like 1, MGST1-Like 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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