The Rat Prolactin (PRL) ELISA Kit offers a cutting-edge solution for the quantitative measurement of Prolactin levels in diverse rat biological samples.
Prolactin, a pivotal hormone with multifaceted roles in reproduction, parental behavior, and metabolic regulation, plays a critical function in the endocrine system. By accurately quantifying Prolactin concentrations, researchers gain valuable insights into its physiological activities and its involvement in various biological processes. Accurate assessment of Prolactin levels is crucial for elucidating its impact on reproductive health, lactation, and metabolic functions in rat models. The Rat PRL ELISA Kit provides researchers with a reliable tool to explore the complex interplay of Prolactin in different physiological pathways, paving the way for a deeper understanding of its regulatory mechanisms and potential therapeutic targets. Engineered with precision and reliability in mind, this ELISA kit ensures exceptional sensitivity and specificity, guaranteeing consistent and reproducible results. Researchers can trust in the robust performance of the Rat PRL ELISA Kit to facilitate their research endeavors and advance scientific discoveries in the field of endocrinology and hormonal regulation.
Product Name:
Rat PRL (Prolactin) ELISA Kit
SKU:
AEES00522
Target:
Rat PRL (Prolactin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PRL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PRL. You can calculate the concentration of Rat PRL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
93-106
101-107
Average (%)
99
101
104
1:4
Range (%)
94-105
90-103
96-110
Average (%)
98
96
104
1:8
Range (%)
85-96
94-108
91-103
Average (%)
90
102
98
1:16
Range (%)
95-108
91-102
90-98
Average (%)
101
98
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
105
EDTA plasma (n=5)
88-99
92
Cell culture media (n=5)
86-97
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
8.9
20.68
83.8
8.89
23.84
81.45
Standard deviation
0.45
1.02
3.61
0.47
1.17
4.23
C V (%)
5.06
4.93
4.31
5.29
4.91
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PRL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PRL in samples. No significant cross-reactivity or interference between Rat PRL and analogues was observed.
