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Rat Presenilin 2 (PS2) ELISA Kit

SKU:
RTDL00871
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O88777
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PSEN2,AD3L,AD4,PS2,STM2,AD3LP,AD5,Alzheimer Disease 4
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Presenilin 2 (PS2) ELISA Kit

The Rat Presenilin-2 (PS2) ELISA Kit is specially designed for the quantitative detection of rat presenilin-2 in samples such as serum, plasma, and tissue homogenates. Presenilin-2 is an integral membrane protein that plays a key role in the processing of amyloid precursor protein (APP) and the production of beta amyloid peptides, which are known to be involved in the pathogenesis of Alzheimer's disease.This ELISA kit offers high sensitivity and specificity, ensuring accurate and reliable results for research purposes.

By measuring presenilin-2 levels, researchers can gain insights into the mechanisms underlying Alzheimer's disease and other neurodegenerative disorders. This kit is a valuable tool for studying presenilin-2 biology and developing potential therapeutic interventions targeting this protein.

Product Name:Rat Presenilin 2 (PS2) ELISA Kit
Product Code:RTDL00871
Size:96 Assays
Target:PS2
Synonyms:PSEN2, AD3L, AD4, PS2, STM2, AD3LP, AD5, Alzheimer Disease 4
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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