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Rat Presenilin 1 (PSEN1) ELISA Kit

SKU:
RTDL00872
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P97887
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FAD,AD3,PS1,PSNL1,S182,Alzheimer Disease 3,Protein S182,Presenilin-1 CTF12
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Rat Presenilin 1 (PSEN1) ELISA Kit

The Rat Presenilin-1 (PSEN1) ELISA Kit is a highly reliable and accurate tool for detecting levels of PSEN1 in rat serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.PSEN1 is a key protein involved in the processing and formation of amyloid-beta peptides, which are implicated in Alzheimer's disease.

By measuring PSEN1 levels using this ELISA kit, researchers can gain valuable insights into the mechanisms underlying neurodegenerative disorders and develop potential therapeutic interventions.Overall, the Rat PSEN1 ELISA Kit offers a powerful tool for studying the role of PSEN1 in disease pathology and for advancing our understanding of Alzheimer's and other related conditions.

Product Name:Rat Presenilin 1 (PSEN1) ELISA Kit
Product Code:RTDL00872
Size:96 Assays
Target:PSEN1
Synonyms:FAD, AD3, PS1, PSNL1, S182, Alzheimer Disease 3, Protein S182, Presenilin-1 CTF12
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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