Rat PPAR-γ (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit (AEES00505)
- SKU:
- AEES00505
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 0.10 ng/mL
- Range:
- 0.16-10 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Product Name: | Rat PPAR-γ (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit |
SKU: | AEES00505 |
Target: | Rat PPAR-γ (Peroxisome Proliferator Activated Receptor Gamma) |
Size: | 96T |
Assay type: | Sandwich-ELISA |
Assay time: | 3.5h |
Sensitivity: | 0.10 ng/mL |
Detection range: | 0.16-10 ng/mL |
Kit component: |
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This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PPAR-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PPAR-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PPAR-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PPAR-γ. You can calculate the concentration of Rat PPAR-γ in the samples by comparing the OD of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | serum, plasma and other biological fluids; 100μL |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of Rat PPAR-γ concentrations in serum, plasma and other biological fluids. |
Specificity: | This kit recognizes Rat PPAR-γ in samples. No significant cross-reactivity or interference between Rat PPAR-γ and analogues was observed. |