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Rat Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) ELISA Kit

SKU:
RTDL00600
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P48548
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Kir3.4,CIR,KATP1,GIRK4,G protein-activated inward rectifier potassium channel 4,Cardiac inward rectifier,Inward rectifier K(+) channel Kir3.4,Heart KATP channel
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
$899
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Description

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Rat Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) ELISA Kit

The Rat KCNJ5 (Potassium Inwardly Rectifying Channel Subfamily J Member 5) ELISA Kit is specifically designed for the precise detection of KCNJ5 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.KCNJ5 is a vital protein that belongs to the inwardly rectifying potassium channel family, playing a crucial role in regulating potassium transport across cell membranes.

Dysregulation of KCNJ5 has been linked to various health conditions, including cardiac arrhythmias, hypertension, and other cardiovascular disorders. As such, this ELISA kit serves as a valuable tool for studying the function and expression of KCNJ5 in rat models, shedding light on its potential implications in disease mechanisms and therapeutic interventions.

Product Name:Rat Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) ELISA Kit
Product Code:RTDL00600
Size:96 Assays
Target:KCNJ5
Synonyms:Kir3.4, CIR, KATP1, GIRK4, G protein-activated inward rectifier potassium channel 4, Cardiac inward rectifier, Inward rectifier K(+) channel Kir3.4, Heart KATP channel
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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