The E2 (Estradiol) ELISA Kit from Assay Genie is designed specifically for the quantitative detection of Estradiol in various biological samples from rats and pigs. Estradiol is a key estrogen hormone that plays a pivotal role in various biological processes, including reproduction, bone health, and metabolic regulation. In both rats and pigs, understanding variables such as Estradiol levels can have significant implications on studies related to reproductive physiology, metabolic disease, and overall organismal health. Accurate measurement of Estradiol is therefore crucial for these research areas.
Assay Genie's E2 (Estradiol) ELISA Kit provides superior sensitivity and specificity, ensuring trustworthy and repeatable results. Produced under rigorous quality control standards, this kit offers robust performance that contributes to the consistency of your experiments. Its user-friendly features make it an excellent option for both research laboratories and clinical settings requiring reliable hormone quantification. Choose Assay Genie's Rat/Porcine E2 (Estradiol) ELISA Kit for precise and reliable detection of this vital hormone in your studies.
Product Name:
Rat/Porcine E2 (Estradiol) ELISA Kit
SKU:
AEES00013
Target:
Rat/Porcine E2 (Estradiol)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
1.00 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. During the reaction, E2 in the sample or standard competes with a fixed amount of E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
82-90
88-108
87-104
Average (%)
85
98
95
1:4
Range (%)
83-98
85-95
81-112
Average (%)
89
89
93
1:8
Range (%)
85-104
80-89
83-92
Average (%)
91
84
85
1:16
Range (%)
81-105
87-101
80-91
Average (%)
87
94
84
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
80-91
84
EDTA plasma (n=5)
82-93
87
Cell culture media (n=5)
85-105
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.33
90.22
200.31
41.25
92.32
201.59
Standard deviation
3.52
6.8
10.67
3.76
7.36
12.55
C V (%)
8.75
7.54
5.33
9.12
7.98
6.23
Sample type &Sample volume:
serum, plasma; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum, plasma.
Specificity:
This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
