Rat PLAUR/uPAR (Plasminogen Activator, Urokinase Receptor) CLIA Kit
The Rat PLAUr (uPAR) Plasminogen Activator & Urokinase Receptor CLIA Kit (RTES00586) is a highly sensitive and specific assay designed for accurate detection of PLAUr levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.PLAUr, also known as uPAR (Urokinase Plasminogen Activator Receptor), is a key component in the plasminogen activation system, playing a crucial role in cell migration, adhesion, and invasion.
Dysregulation of PLAUr expression has been implicated in various diseases, including cancer, inflammation, and fibrosis, making it a valuable biomarker for studying these conditions and potential therapeutic targets.Investigate the role of PLAUr in disease pathology and drug development with the Rat PLAUr (uPAR) Plasminogen Activator & Urokinase Receptor CLIA Kit (RTES00586), offering accurate and precise measurement of PLAUr levels in rat samples.
Product Name:
Rat PLAUR/uPAR (Plasminogen Activator, Urokinase Receptor) CLIA Kit
SKU:
RTES00586
Target:
Rat PLAUR/uPAR (Plasminogen Activator, Urokinase Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PLAUR/uPAR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PLAUR/uPAR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PLAUR/uPAR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PLAUR/uPAR. You can calculate the concentration of Rat PLAUR/uPAR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
100-113
98-109
Average (%)
106
106
104
1:4
Range (%)
98-115
91-104
98-110
Average (%)
105
96
104
1:8
Range (%)
87-98
89-102
101-115
Average (%)
92
96
106
1:16
Range (%)
101-117
96-109
99-113
Average (%)
108
102
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
107
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.88
123.42
425.9
50.69
124.26
422.94
Standard deviation
4.48
9.63
48.04
5.02
10.26
44.11
C V (%)
9.17
7.8
11.28
9.9
8.26
10.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PLAUR/uPAR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PLAUR/uPAR in samples. No significant cross-reactivity or interference between Rat PLAUR/uPAR and analogues was observed.
