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Rat Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit

SKU:
RTDL00811
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q05028
Sensitivity:
12.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
PDGF-B,PDGF2,SIS,SSV,C-sis,Simian Sarcoma Viral(v-sis)Oncogene Homolog,Platelet Derived Growth Factor Beta Polypeptide,Proto-oncogene c-Sis,Becaplermin
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

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Rat Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit

The Rat Platelet-Derived Growth Factor Subunit B (PDGF-B) ELISA Kit is specially designed for the accurate measurement of PDGF-B levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.PDGF-B is a key growth factor that plays a crucial role in cell proliferation and tissue repair processes. It is involved in a wide range of physiological and pathological conditions, including wound healing, tissue regeneration, and tumor growth.

Therefore, accurate detection of PDGF-B levels is essential for studying these processes and developing potential therapeutic interventions.Overall, the Rat PDGF-B ELISA Kit is a valuable tool for researchers looking to investigate the role of PDGF-B in various biological processes and diseases, providing reliable and reproducible data for their experiments.

Product Name:Rat Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit
Product Code:RTDL00811
Size:96 Assays
Target:PDGFB
Synonyms:PDGF-B, PDGF2, SIS, SSV, C-sis, Simian Sarcoma Viral(v-sis)Oncogene Homolog, Platelet Derived Growth Factor Beta Polypeptide, Proto-oncogene c-Sis, Becaplermin
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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