Rat Platelet Derived Growth Factor D (PDGFD) ELISA Kit
The Rat Platelet-Derived Growth Factor D (PDGF-D) ELISA Kit is specifically designed for the accurate and sensitive detection of PDGF-D levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this ELISA kit provides reliable and reproducible results, making it an invaluable tool for a variety of research applications.PDGF-D is a key growth factor that plays a crucial role in various cellular processes, including cell growth, proliferation, and differentiation. It is involved in numerous physiological and pathological processes, such as wound healing, tissue repair, and cancer development.
The ability to accurately quantify PDGF-D levels in rat samples can provide valuable insights into the mechanisms underlying these processes and aid in the development of novel therapeutic strategies.Overall, the Rat PDGF-D ELISA Kit from Assay Genie is an essential tool for researchers studying the role of PDGF-D in various biological processes and disease conditions in rats. Its high sensitivity, specificity, and reliability make it an indispensable asset for advancing scientific knowledge and potentially improving clinical outcomes.
Product Name:
Rat Platelet Derived Growth Factor D (PDGFD) ELISA Kit
Product Code:
RTDL00812
Size:
96 Assays
Target:
PDGFD
Synonyms:
PDGF-D, IEGF, SCDGF-B, Spinal Cord Derived Growth Factor B, Iris-expressed growth factor, Platelet-derived growth factor D, receptor-binding form latent form
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
