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Rat Plasminogen Activator, Tissue (tPA) ELISA Kit

SKU:
RTDL01054
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19637
Sensitivity:
0.124ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
PLAT,T-PA,t-plasminogen activator,Alteplase,Reteplase
Reactivity:
Rat
Sample Type:
Plasma
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Plasminogen Activator, Tissue (tPA) ELISA Kit

The Rat Plasminogen Activator Tissue (tPA) ELISA Kit is specifically designed for the accurate measurement of tPA levels in rat serum, plasma, and tissue samples. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research purposes.tPA is a critical enzyme involved in fibrinolysis, breaking down blood clots and maintaining proper blood flow. Elevated levels of tPA have been associated with various cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.

This ELISA kit provides researchers with a reliable tool to investigate the role of tPA in disease processes and evaluate the efficacy of potential therapeutic interventions. With its user-friendly protocols and high performance characteristics, the Rat tPA ELISA Kit from Assaygenie is an essential tool for advancing research in the field of cardiovascular health.

Product Name:Rat Plasminogen Activator, Tissue (tPA) ELISA Kit
Product Code:RTDL01054
Size:96 Assays
Target:tPA
Synonyms:PLAT, T-PA, t-plasminogen activator, Alteplase, Reteplase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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