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Rat Pim-1 Oncogene (PIM1) ELISA Kit (RTDL00832)

SKU:
RTDL00832
Product Type:
ELISA Kit
Sensitivity:
0.068ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Proto-Oncogene Serine/Threonine-Protein Kinase Pim-1
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749

Description

system_update_altDatasheet

Rat Pim-1 Oncogene (PIM1) ELISA Kit

The Rat PIM-1 Oncogene (PIM1) ELISA Kit is a powerful tool for the precise measurement of PIM1 levels in rat samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it a valuable asset for various research applications.PIM1 is a key oncogene that plays a vital role in cancer development and progression by regulating cell proliferation and survival pathways.

Understanding the expression levels of PIM1 is crucial for studying tumorigenesis and identifying potential targets for cancer therapy. The Rat PIM-1 Oncogene ELISA Kit provides researchers with a reliable method to investigate the involvement of PIM1 in cancer biology and explore its therapeutic implications.

Product Name:Rat Pim-1 Oncogene (PIM1) ELISA Kit
Product Code:RTDL00832
Size:96 Assays
Target:PIM1
Synonyms:Proto-Oncogene Serine/Threonine-Protein Kinase Pim-1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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