Rat Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit
The Rat Phosphodiesterase 3A (cGMP-inhibited) ELISA Kit is a powerful tool for the precise measurement of PDE3A levels in rat samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it well-suited for various research purposes.Phosphodiesterase 3A is an enzyme that plays a key role in regulating cGMP levels in cells, impacting processes such as vascular smooth muscle relaxation and platelet aggregation. Dysregulation of PDE3A has been linked to various diseases, including cardiovascular disorders and diabetes, underscoring its importance as a potential therapeutic target.
With the Rat Phosphodiesterase 3A (cGMP-inhibited) ELISA Kit, researchers can explore the mechanisms underlying PDE3A function, identify potential drug candidates, and advance our understanding of related pathologies. This kit offers a reliable and efficient solution for studying PDE3A in a rat model system, facilitating groundbreaking discoveries in the field of molecular biology.
Product Name:
Rat Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit
Product Code:
RTDL00809
Size:
96 Assays
Target:
PDE3A
Synonyms:
CGI-PDE, Cyclic GMP-inhibited phosphodiesterase A, cGMP-inhibited 3',5'-cyclic phosphodiesterase A
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
