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Rat Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) ELISA Kit

SKU:
RTDL00808
Product Type:
ELISA Kit
Sensitivity:
0.042ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Hcam3,Cam-PDE 1C,Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
$899
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Description

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Rat Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) ELISA Kit

The Rat Phosphodiesterase 1C (Calmodulin-dependent PDE1C) ELISA Kit is a powerful tool for accurately measuring levels of PDE1C in rat samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.PDE1C is a key enzyme involved in cellular signaling pathways, specifically in the regulation of cyclic nucleotide levels. Dysregulation of PDE1C has been implicated in various diseases, including cardiovascular disorders, neurological conditions, and metabolic syndromes.

Therefore, the Rat PDE1C ELISA Kit is an indispensable resource for studying these diseases and potential therapeutic interventions.Overall, the Rat Phosphodiesterase 1C (Calmodulin-dependent PDE1C) ELISA Kit is an essential tool for researchers looking to accurately quantify PDE1C levels in rat samples, providing valuable insights into the role of this enzyme in health and disease.

Product Name:Rat Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) ELISA Kit
Product Code:RTDL00808
Size:96 Assays
Target:PDE1C
Synonyms:Hcam3, Cam-PDE 1C, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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