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Rat Phosphatase And Tensin Homolog (PTEN) ELISA Kit

SKU:
RTDL00875
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O54857
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
BZS,MHAM,MMAC1,PTEN1,TEP1,Mutated In Multiple Advanced Cancers 1,Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Phosphatase And Tensin Homolog (PTEN) ELISA Kit

The Rat Phosphatase and Tensin Homolog (PTEN) ELISA Kit is a powerful tool for the precise measurement of PTEN levels in rat samples. This kit provides exceptional sensitivity and specificity, guaranteeing consistent and accurate results for various research applications.PTEN is a key tumor suppressor gene that regulates cell growth, proliferation, and survival.

Dysregulation of PTEN has been linked to various cancers and other diseases, highlighting its importance as a potential therapeutic target and biomarker.With the Rat PTEN ELISA Kit, researchers can confidently study PTEN levels in rat serum, plasma, and tissue samples, paving the way for important discoveries in cancer research and beyond.

Product Name:Rat Phosphatase And Tensin Homolog (PTEN) ELISA Kit
Product Code:RTDL00875
Size:96 Assays
Target:PTEN
Synonyms:BZS, MHAM, MMAC1, PTEN1, TEP1, Mutated In Multiple Advanced Cancers 1, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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