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Rat PF4 (Platelet Factor 4) ELISA Kit

SKU:
AEES00511
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
0.10 ng/mL
Range:
0.16-10 ng/mL
Sample Type:
Serum, plasma and other biological fluids
€499
Frequently bought together:

Description

Rat PF4 (Platelet Factor 4) ELISA Kit

The Rat PF4 (Platelet Factor 4) ELISA Kit is a specialized assay designed for the quantitative detection of Platelet Factor 4 (PF4) levels in rat samples.

PF4 is a protein released from the alpha-granules of activated platelets and has a crucial role in various biological processes, including promoting platelet aggregation, mediating inflammatory responses, and modulating endothelial cell function. This ELISA kit offers researchers a reliable method to measure PF4 concentrations accurately, which can provide valuable insights into platelet activation, thrombosis, and inflammatory pathways. Accurate measurement of PF4 levels using this ELISA kit is essential for studying platelet function, understanding its implications in the coagulation cascade, and investigating its involvement in various thrombotic disorders or inflammatory conditions. The quantitative data obtained from this ELISA can contribute to a better understanding of PF4's role in both physiological and pathological conditions, facilitating research in areas such as hemostasis, thrombosis, and vascular biology. The Rat PF4 ELISA Kit by Assay Genie ensures exceptional sensitivity and specificity, enabling precise and reproducible results. Manufactured under strict quality control measures, this kit offers robust performance, ease of use, and reliable data output, making it an excellent choice for researchers investigating platelet biology, thrombotic disorders, and related fields of study.

Product Name:Rat PF4 (Platelet Factor 4) ELISA Kit
Product Code:AEES00511
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:0.16-10 ng/mL
Sensitivity:0.10 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat PF4 in samples. No significant cross-reactivity or interference between Rat PF4 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat PF4 concentrations in Serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PF4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PF4. You can calculate the concentration of Rat PF4 in the samples by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat PF4 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat PF4 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (ng/mL)0.521.313.470.501.413.72
Standard deviation0.030.050.130.030.080.20
CV (%)5.773.823.756.005.675.38

Recovery

The recovery of Rat PF4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)89-10094
EDTA plasma (n=8)92-108100
Cell culture media (n=8)95-109101
Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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