Rat PDGFA (Platelet Derived Growth Factor Subunit A) CLIA Kit
The Rat PDGFA (Platelet-Derived Growth Factor Subunit A) CLIA Kit is a highly sensitive and specific assay designed for the precise quantification of PDGFA levels in rat serum, plasma, and tissue culture supernatants. This kit provides accurate and reproducible results, making it an essential tool for researchers studying cell growth, proliferation, and differentiation.PDGFA is a key growth factor that plays a crucial role in regulating cell growth and division, particularly in the formation of blood vessels.
Dysregulation of PDGFA has been implicated in various diseases, including cancer and cardiovascular disorders, highlighting its importance as a potential therapeutic target.By using the Rat PDGFA CLIA Kit, researchers can accurately measure PDGFA levels in rat samples, facilitating the study of PDGFA signaling pathways and the development of novel therapeutic strategies. This kit is an invaluable asset for any laboratory investigating the role of PDGFA in physiological and pathological processes.
Product Name:
Rat PDGFA (Platelet Derived Growth Factor Subunit A) CLIA Kit
SKU:
RTES00448
Target:
Rat PDGFA (Platelet Derived Growth Factor Subunit A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGF-A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PDGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PDGF-A. You can calculate the concentration of Rat PDGF-A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
100-116
84-98
Average (%)
98
108
90
1:4
Range (%)
98-112
90-100
101-115
Average (%)
105
95
106
1:8
Range (%)
90-101
100-116
90-104
Average (%)
95
107
97
1:16
Range (%)
98-112
92-102
99-116
Average (%)
104
97
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
92
EDTA plasma (n=5)
90-103
98
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.92
53.8
206.97
21.46
53.71
208.61
Standard deviation
2.63
5.34
20.32
2.07
5.9
16.73
C V (%)
11.47
9.93
9.82
9.65
10.98
8.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PDGF-A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PDGF-A in samples. No significant cross-reactivity or interference between Rat PDGF-A and analogues was observed.
