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Rat Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA Kit

SKU:
RTDL00851
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D3Z9J9
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ADPN,C22orf20,iPLA(2)epsilon,Adiponutrin,Acylglycerol O-acyltransferase,Calcium-independent phospholipase A2-epsilon
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

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Rat Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA Kit

The Rat PNPLA3 (Patatin Like Phospholipase Domain Containing Protein 3) ELISA Kit is specifically designed for the accurate detection of PNPLA3 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PNPLA3 is a critical protein involved in lipid metabolism and has been linked to liver diseases such as non-alcoholic fatty liver disease (NAFLD) and alcoholic liver disease.

Detecting PNPLA3 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Rat PNPLA3 ELISA Kit is an essential tool for researchers studying lipid metabolism, liver diseases, and related conditions in rats, offering precise and accurate measurements for in-depth analysis and investigation.

Product Name:Rat Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA Kit
Product Code:RTDL00851
Size:96 Assays
Target:PNPLA3
Synonyms:ADPN, C22orf20, iPLA(2)epsilon, Adiponutrin, Acylglycerol O-acyltransferase, Calcium-independent phospholipase A2-epsilon
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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