The Rat PAP (Plasmin-Antiplasmin) Complex CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of the PAP complex in rat samples. This kit is ideal for measuring levels of the PAP complex in serum, plasma, and other biological samples, providing researchers with reliable and reproducible results for a wide range of research applications.The PAP complex is an important biomarker in the field of coagulation and fibrinolysis, reflecting the balance between plasminogen activation and plasmin inhibition.
Changes in PAP levels have been associated with various pathologies, including thrombotic disorders, liver disease, and inflammation. Studying the PAP complex can provide valuable insights into these diseases and help in the development of potential therapeutic interventions.With its high sensitivity and specificity, the Rat PAP Complex CLIA Kit is a valuable tool for researchers looking to explore the role of the PAP complex in health and disease. Get accurate and reliable results with this advanced assay kit from Assay Genie.
Product Name:
Rat PAP (Plasmin-Antiplasmin Complex) CLIA Kit
SKU:
RTES00441
Target:
Rat PAP (Plasmin-Antiplasmin Complex)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PAP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PAP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PAP. You can calculate the concentration of Rat PAP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-112
86-98
90-104
Average (%)
106
93
96
1:4
Range (%)
96-111
93-104
86-98
Average (%)
103
98
93
1:8
Range (%)
101-115
99-115
101-116
Average (%)
107
107
107
1:16
Range (%)
91-107
102-116
96-113
Average (%)
98
108
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
86-102
93
Cell culture media (n=5)
98-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.55
12.66
37.07
5.24
11.91
34.17
Standard deviation
0.48
1.34
3.47
0.62
1.12
2.73
C V (%)
8.65
10.58
9.36
11.83
9.4
7.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PAP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PAP in samples. No significant cross-reactivity or interference between Rat PAP and analogues was observed.
