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Rat PAI1 (Plasminogen Activator Inhibitor 1) ELISA Kit (AEES00539)

SKU:
AEES00539
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
0.94 ng/mL
Range:
1.56-100 ng/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Rat PAI1 (Plasminogen Activator Inhibitor 1) ELISA Kit

The Rat Plasminogen Activator Inhibitor 1 (PAI-1) ELISA Kit is a specialized assay designed for the quantitative measurement of PAI-1 levels in rat biological samples.

PAI-1, as a critical regulator of the fibrinolytic system, plays a key role in inhibiting tissue plasminogen activators and regulating the breakdown of blood clots. Monitoring PAI-1 levels provides valuable insights into fibrinolysis, clotting disorders, and various pathophysiological conditions associated with thrombosis and hemostasis. This ELISA kit offers researchers a reliable and accurate method to quantify PAI-1 concentrations, enabling investigations into the dynamics of the fibrinolytic system, coagulation cascades, and thrombotic events in rat models. By precisely measuring PAI-1 levels, researchers can better understand fibrinolytic processes, clot formation, and the underlying mechanisms of thrombotic diseases. Manufactured with stringent quality control standards, the Rat PAI-1 ELISA Kit ensures exceptional sensitivity and specificity, allowing for reproducible and reliable results. This kit provides robust performance and ease of use, making it an excellent choice for research studies focused on fibrinolysis, coagulation disorders, thrombosis, and other related fields in rat models.

Product Name:Rat PAI1 (Plasminogen Activator Inhibitor 1) ELISA Kit
Product Code:AEES00539
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:1.56-100 ng/mL
Sensitivity:0.94 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat PAI1 in samples. No significant cross-reactivity or interference between Rat PAI1 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat PAI1 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PAI1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PAI1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PAI1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PAI1. You can calculate the concentration of Rat PAI1 in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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