Rat PⅢNP (N-Terminal Procollagen Ⅲ Propeptide) ELISA Kit
The Rat N-Terminal Procollagen Type III Propeptide (PⅢNP) ELISA Kit is a specialized assay designed for the quantitative measurement of N-terminal propeptide of type III procollagen (PⅢNP) levels in rat biological samples. PⅢNP is a clinically significant collagen biomarker associated with collagen synthesis and turnover, particularly in the context of extracellular matrix remodeling, fibrosis, and tissue repair processes. Monitoring PⅢNP levels provides valuable information on collagen metabolism, fibrotic conditions, and connective tissue health. This ELISA kit offers researchers a precise and reliable method to quantify PⅢNP concentrations, facilitating investigations into collagen-related processes, fibrotic disorders, wound healing, and tissue regeneration in rat models.
By accurately measuring PⅢNP levels, researchers can gain insights into fibrosis progression, connective tissue disorders, and potential therapeutic interventions targeting collagen turnover and extracellular matrix dynamics. Manufactured with stringent quality control measures, the Rat PⅢNP ELISA Kit ensures exceptional sensitivity and specificity, enabling reproducible and accurate results. This kit provides researchers with robust performance, ease of use, and the reliability needed for in-depth studies of collagen metabolism and related pathologies in preclinical rat models, making it an excellent tool for research and scientific investigations.
Product Name:
Rat PⅢNP (N-Terminal Procollagen Ⅲ Propeptide) ELISA Kit
SKU:
AEES00538
Target:
Rat PⅢNP (N-Terminal Procollagen Ⅲ Propeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PⅢNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PⅢNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PⅢNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PⅢNP. You can calculate the concentration of Rat PⅢNP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-107
93-104
82-95
Average (%)
103
98
90
1:4
Range (%)
87-97
95-104
89-97
Average (%)
92
100
93
1:8
Range (%)
101-108
97-104
99-109
Average (%)
105
100
103
1:16
Range (%)
93-106
95-109
94-106
Average (%)
99
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-109
101
EDTA plasma (n=5)
91-101
96
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.58
221.8
943.75
87.18
235.85
864.41
Standard deviation
5.66
10.98
50.59
5.11
11.13
35.79
C V (%)
5.68
4.95
5.36
5.86
4.72
4.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PⅢNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PⅢNP in samples. No significant cross-reactivity or interference between Rat PⅢNP and analogues was observed.
