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Rat Oxoglutarate Dehydrogenase (OGDH) ELISA Kit

SKU:
RTDL00784
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q5XI78
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AKGDH,E1k,OGDC,Alpha-Ketoglutarate Dehydrogenase,Lipoamide,2-Oxoglutarate Dehydrogenase E1 Component,Mitochondrial
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
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Description

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Rat Oxoglutarate Dehydrogenase (OGDH) ELISA Kit

The Rat Oxoglutarate Dehydrogenase (OGDH) ELISA Kit is specifically designed for the precise quantification of OGDH levels in rat serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable tool for various research applications.OGDH is a key enzyme involved in the tricarboxylic acid (TCA) cycle, playing a crucial role in energy production and metabolism.

Dysregulation of OGDH activity has been linked to various metabolic disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Whether investigating metabolic disorders, studying cellular metabolism, or exploring potential therapeutic targets, the Rat OGDH ELISA Kit provides researchers with a reliable and efficient method for quantifying OGDH levels in rat samples.

Product Name:Rat Oxoglutarate Dehydrogenase (OGDH) ELISA Kit
Product Code:RTDL00784
Size:96 Assays
Target:OGDH
Synonyms:AKGDH, E1k, OGDC, Alpha-Ketoglutarate Dehydrogenase,Lipoamide, 2-Oxoglutarate Dehydrogenase E1 Component,Mitochondrial
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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