Rat OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
The Rat OxLDL (Oxidized Low-Density Lipoprotein) ELISA Kit is expertly crafted to facilitate the precise measurement of Oxidized LDL levels in diverse rat biological specimens. OxLDL, a modified version of LDL cholesterol, is acclaimed for its pivotal role in atherogenesis and cardiovascular disorders. As an influential pro-atherogenic factor, OxLDL is involved in a myriad of pathophysiological processes, including endothelial dysfunction, foam cell formation, and inflammation within the arterial walls. Accurate quantification of Oxidized LDL using this ELISA kit offers invaluable insights into the mechanisms underlying atherosclerosis and related cardiovascular complications.
Understanding the impact of OxLDL on lipid metabolism, vascular health, and atherosclerotic plaque development is crucial for unveiling novel therapeutic strategies and diagnostic approaches in the realm of cardiovascular research. The Rat OxLDL (Oxidized Low-Density Lipoprotein) ELISA Kit from Assay Genie guarantees exceptional sensitivity and specificity, ensuring precise and reproducible outcomes. With a commitment to stringent quality control standards, this kit delivers robust performance and user-friendly operation, making it a prime choice for cutting-edge research in cardiovascular health and disease.
Product Name:
Rat OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
SKU:
AEES00502
Target:
Rat OxLDL (Oxidized Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OxLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OxLDL. You can calculate the concentration of Rat OxLDL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
90-106
93-107
Average (%)
103
97
99
1:4
Range (%)
100-113
81-95
96-112
Average (%)
107
87
102
1:8
Range (%)
96-108
83-93
90-107
Average (%)
103
88
98
1:16
Range (%)
100-113
84-96
93-104
Average (%)
107
90
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
102
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.6
2.6
12.2
1.5
2.5
13.0
Standard deviation
0.1
0.1
0.5
0.1
0.1
0.4
C V (%)
6.25
3.85
4.1
6.67
4.0
3.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat OxLDL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat OxLDL in samples. No significant cross-reactivity or interference between Rat OxLDL and analogues was observed.
